Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line | Cell search engine | Slide search engine | Lysate search engine

Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Name	Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26273
Category	Cell Line
Prices	$1,099.00
Sizes	1 vial
Applications	WB FC
Gene	TLR10
Species	Human
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR10 reporter line may be useful for TLR10-based functional studies. The functional activity of the NF-kB/SEAP reporter in the TLR10 stable cell line has been established and validated through PMA activation of the NF-kB pathway (Figure 1). Hence, the cell line may be useful for reseasrchers screening for putative TLR10 ligands activating the NF-kB pathway. Any specific ligand for TLR10 is still unknown. However, TLR10 can indeed activate the NF-kB signaling pathway through direct association with the common Toll/IL-1 receptor domain adapter, MyD88, according to a recent study utilizing a chimeric CD4/TLR10 molecule. TLR10 expression in the TLR10/NF-kB/SEAPorter HEK 293 cell line has been validated by Western blotting (Fig. 2) and flow cytometry (Fig. 3). Contents: 3~4 x 10^6 cells Biosafety Level: 2
Supplier Page	Shop

Product images

Western Blot: Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26273] - Analysis of TLR10 expression in the TLR10 reporter cell line using an HA antibody (20 ug total protein/lane). Legend: Cont: Control, NF-kB/SEAPorterTM HEK 293 cell line; TLR10: TLR10 reporter cell line.

Flow Cytometry: Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26273] - Analysis of TLR10. Cell surface expression of TLR10 in TLR10 reporter cell line was analyzed by flow cytometry using a FITC-conjugated TLR10 antibody and compared with the NF-kB/SEAPorterTM HEK 293 cell line. Mouse IgG1 isotype control and Cell Surface TLR Staining Flow Kit were used for this test.

Ligand Activation: Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26273] - TLR10 reporter cells were plated in a 96-well plate at 5 x 10^4 cells per well. Cells were stimulated with various concentrations of phorbol 12-myristate 13-acetate (PMA), which is a positive control for NF-kB induction for 24 h. SEAP was analyzed using the SEAPorter Assay Kit.